Flag tag polyclonal antibody
來源:
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作者:jienitai
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發布時間: 2019-03-11
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781 次瀏覽
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This antibody is produced by immunizing rabbits with a synthetic peptide DYKDDDDK (KLH-coupled).
Flag tag polyclonal antibody
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貨號
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規格
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價格（元）
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貨期
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GAB800501
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100ul
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￥600.00
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2~3天
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GAB800502
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200ul
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￥900.00
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2~3天
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GAB800503
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1000ul
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詢價
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2~3天
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Background
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The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which
does not appear to interfere with the bioactivity or the biodistribution
of the recombinant protein. It has been used extensively as a general
epitope tag in expression vectors. It can be used for affinity
chromatography, then used to separate recombinant, overexpressed protein
from wild-type protein expressed by the host organism. It can also be
used in the isolation of protein complexes with multiple subunits.A
Flag-tag can be used in many different assays that require recognition
by an antibody. If there is no antibody against the studied protein,
adding a Flag-tag to this protein allows one to follow the protein with
an antibody against the Flag sequence.
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Description
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The Flag tag antibody can recognize C-terminal, internal, and N-terminal
Flag-tag
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Source
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This antibody is produced by immunizing rabbits with a synthetic peptide
DYKDDDDK (KLH-coupled).
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Applications
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WB 1:1000-2000, ICH 1:500-2000. Optimal dilution has to be determined by
the user.
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Applicable Species
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Mouse, rat, human.
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Specifications
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Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5% BSA
with 0.05% sodium azide. Antibody was purified by affinity
chromatography.
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Storage conditions
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Store
at 2-8℃ for 3 months, -20℃ for 1 year. To avoid freeze-thaw cycles,
reconstituted antibody should be aliquoted before freezing for
short-term storage (-20℃) or for long-term storage (-80℃).
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